O-299 Sperm DNA fragmentation by TUNEL influences live birth in egg recipients after euploid embryo transfer

Abstract Study question Does sperm DNA fragmentation (SDF) by TUNEL affect reproductive success measured as live birth rate (LBR) in egg recipients after euploid embryo transfer? Summary answer SDF increases miscarriage rates with PGT-A. Therefore, depends on damage transfer. What is known already Sperm integrity important for optimal fertilisation, implantation and pregnancy. Although controversial, several studies have shown that correlates adverse pregnancy outcomes infertile men. Ooplasmic repair mechanisms may occur female gametes, mainly young women. However, research the impact of LBR are limited. Also, chromosomally normal embryos analysed PGT-A offers opportunity to isolate factor study male factor. The aim this assess whether high level semen has an terms rate. design, size, duration This a retrospective 467 cycles recipients. population consists couples transfer (February 2017-November 2022). Semen samples were obtained measure from 447 was performed 1831 blastocysts. Trophectoderm biopsies (d5/d6 blastocysts) NGS (Veriseq Illumina). Embryos frozen 568 blastocysts transferred subsequent cycle. Conventional hormone replacement used endometrial preparation recipient patients. Participants/materials, setting, methods Cohort attending private fertility clinic undergoing ART their autologous semen, donated oocytes Terminal-deoxyribonucleotidyl-ARTSferase-mediated dUTP Nodule End Labelling (TUNEL) assay using FITC-labelled-dUTP situ cell death detection kit (Roche). cohort divided into two groups according index (DFI): group (DFI>20%) (DFI≤20%). To evaluate Chi-square Linear regression tests used. Main results role chance Of included (average paternal age 41.29 + 7.32 years), 66.5% partners had parameters. We reported 8% Overall, aneuploidy 31.7%. For further statistical analysis confounding factors such as, age, quality, day biopsy included. showed 32.1% 30.3% (p = 0.453). Regarding clinical data, overall 34.9%. There no significant difference between elevate respect biochemical (4.3% vs 7.6%, p 0.799). not differences groups, lower near reach significance found (20.0% 34.4%, 0.050). Pregnancy significantly (26.1% 44.9%, 0.042; 21.7% 31.3%, 0.044;), respectively. higher (40% 12.5%, 0.014). (13.0% 32.6%, 0.024). Limitations, reasons caution Larger including number needed confirm correlation observed eggs fragmentation. Those patients DFI than control values should be treated antioxidants. analyzed treatment Wider implications findings Our suggest influence data show decreased confirms published literature showing affected Trial registration Not Applicable